you were James Bond and were ordered to kill half the population of a city of two million, without notice and without the resources of a major power at your command, what would you do? Do another Hiroshima? No. You will take just a gram or two of a toxin called botulin and put it in the water supply system of the city.The LD50 (amount required for killing 50 per cent of a group) of botulin is 0.6 nanograms per kg weight of a person (1 nanogram is 1 billionth of a gram). And there will be no damage to property! Further, as botulin is a protein and all proteins decay sooner or later, the water contaminated with it will become potable in a while. Small wonder, botulin is one of the most powerful biological weapons. Such weapons have the following advantages.
They are easy and inexpensive to manufacture, weaponise and deliver. They have a long shelf life and are virtually impossible to detect and, therefore, verify; one can store enough biological weapons that will kill the entire population of the world many times over, in just a few small refrigerators or freezers; this is probably what Saddam Hussein did.
One has a wide range of choice, from agents that will lead to virtually cent percent mortality, to agents that will lead to little mortality but high morbidity; or from agents that would have an immediate effect, to agents that will have a delayed effect (silent warfare!). One can develop ethnic-specific weapons. For example, those that will kill or hurt only Americans but not Indians.
Biological weapons can be either live bacteria, fungi (specially for plants) and viruses or toxins. The former category has a potential of multiplying after the organism is released and thus causing far more extensive damage over a long periods of time than the latter.
Today’s repertoire of live biological weapons includes (where not obvious, parenthesis gives the disease caused by the bacterium, virus or rickettsia): Chlamydia peittaci (Influenza psittacosis); Yellow fever virus; Dengue fever virus; Chikungunya virus; O’nyong-nyong virus; Mayaro virus; Ross River virus; Venezuelan equine encephalitis virus; Western equine encephalitis virus; Tick-borne encephalitis virus; Kyasanur Forest Disease virus; Rift Valley fever virus; Junin and other similar viruses (Argentinan haemorrhagic fever); Hantaan virus (Korean haemorrhagic fever); Lassa fever virus; Sindbis virus; Marburg virus; Congo Crimean virus (African haemorrhagic fever); Ebola virus; Variola virus (small pox); Vibrio cholarae (cholera); Salmonella typhose (typhoid); Shigella (dysentry); Francisella tularensis (tularemia); Brucella species; Clostridium tetani (tetanus); Clostridium perfringens (gangrene); Pasteurella pestis (plague); Bacillus anthracis (anthrax); Antinobacillus mallei (glanders); Rickettsia prowazakii (epidemic typhus); Rickettsia tsutsugamushi (scrub typhus); Coxiella burnetii (G-fever); Rickettsia rickettsii (Rocky Mountain spotted fever).
One needs to be infected with only 25 tularemia-causing microorganisms to run the risk of death. The toxins produced or studied as potential biological warfare agents are: Botulin (Clostridium botulinum toxin A); Enterotoxin B from Staphylococcus aureus; Saxitoxin (shellfish poison); Cobrotoxin; Crotoxin (from South American rattle snake); Myotoxin; Cardiotoxin; Bungarotoxin; Aflatoxin; Snail conotoxin; Scorpion toxins; Ricin (derived from castor beans); Substance P; Tetanus toxin; Trichothecene mycotoxins; Shiga toxin (from Shigella dysenteriae or S flexneri); Epsilon toxin from Clostridium perfringens).
And then there are fungi such as Puccinia graminis (black-stem rust of cereals) and Pyricularia oryzae (rice blast) which can destroy whole agriculture fields when sprayed over them in very small amounts. Before the collapse of their empire in 606 B C, the Assyrians used an ingenious method of poisoning their enemy. Rye, widely used at that time, is liable to attack by a poisonous fungus, Claviceps purpurea, which grows in place of the grain and forms a horny mass called ergot. Eating rye bread contaminated with ergot can cause gangrene, abortion and hallucinations.
The Assyrians used this rye-ergot to poison their enemy. The ancient Romans threw carrions into wells to poison the drinking water of their adversaries. In 1347, the Tartars catapulted the bodies of bubonic-plague victims over the city walls of Kaffa, a Black Sea port that served as a gateway to the silk-trade route — a manoeuvre that worked.
In 1942, the Soviets infected the German occupation troops with the Tularemia-causing agent, which eventually led to more that 100,000 cases of the disease on both sides. Between 1936 and 1945, the Japanese Military Unit-731 experimented with biological weapons on the Chinese at PingFan in Manchuria, killing 3,559 prisoners of war with agents like anthrax, cholera, plague and dysentery. The Japanese also released plague on the Chinese civilian population of Chekiang province on several occasions by dropping from aeroplanes fleas fed on infected rats. In fact, China was plagued by diseases from Japan’s biological weapons (typhus, bubonic plague, cholera and anthrax) between 1940 and 1950.
In 1978, Soviet intelligence agents used ricin to murder Georgi Markov, a defector from Bulgaria. In 1979, an accidental release of anthrax from the Soviet bioweapons facility in Sverdlovsk killed some 100 people and much livestock. In 1984, Salmonella was released by the cult followers of Bhagwan Rajneesh in salad bars in four restaurants in The Dalles in Oregon, US, which made 750 people ill; the objective was apparently, to keep voters from the polls to influence a local election! And between 1990 and 1995, the Japanese cult, Aum Shinrikyo, made several unsuccessful attempts to use biological weapons including botulin.
In 1763, the Whites in the US used blankets and handkerchiefs infected with small pox virus against the Red Indians; this led to the death of 6 million original American Indians.
In 1955, US scientists sprayed Q-fever bacteria over Utah in a slurry on human test subjects; not only were they infected but also soldiers on the road blocks! The US used the pig plague-causing organism in Cuba during the Bay of Pigs conflict. And the Anthrax attack in the US just after 9/11 was almost a contained act of biological warfare.
Advances in modern biology have opened up avenues for making designer biological weapons which would, say, exploit genetic or ethnic differences. For example, in the US, those who are above 50 have a depleted immune response. They would thus be far more susceptible to small doses of certain toxic antigens (living organisms or chemicals) which would have no effect on the adult Indian population. Proper release of these antigens in the environment could cause at least temporary disability amongst the Americans over 50, while not affecting Indians. Indeed, when it comes to developing and using biological weapons it is essentially a battle of wits — something in which, perhaps, the deprived section of the world has an advantage, as they have been in any case living by their wits!
The Soviets have developed genetically modified Legionella bacteria that have been shown to induce auto-immunity to myelin (an important component of brain) in mice; when infected with this bacteria the mice die a horrible death.
In 2002, a group of Australian gene engineers accidentally created a mouse virus that kills every one of its victims by wrecking their immune response — something like what HIV does. There would be, as of today, no defence against such a human virus.
The question whether SARS was being developed by China as a biological warfare agent and happened to leak out of the laboratory, has never been satisfactorily answered.
In spite of their being signatories to the Biological Weapons Convention, at least the UK, the US, Russia, Canada, Germany, South Africa, Japan, Iraq, Iran, Syria and North Korea have had extensive biological weapons development and testing programmes — in some cases for at least 80 years.
When, during the Iraq-Kuwait conflict from January 16 to February 20, 1991, Saddam Hussein said that he had the final weapon, several of us had predicted that he had biological weapons such as botulin or anthrax spores which could be put on a Scud war head, even though Iraq had initially denied that it had a biological warfare programme.
On May 31, 1991, the distinguished American scientist, Mathew Meselson, and this writer were invited to address the ambassadors in Geneva, at Chateau de Bossey on Lake Geneva, under the auspices of a residential conference on biological weapons. During a lecture that evening, this writer mentioned about Saddam Hussein having biological weapons. Immediately after the meeting, the organisers introduced me to two German gentlemen who had set up the biological weapons factories in Iraq! These were the factories unearthed later by the CIA.
Subsequently, Iraq declared it had 157 aerial bombs and 25 warheads with botulin, anthrax spores and aflatoxin, the first two of which are the most fatal biological weapons known. An area of 18 sq km. which had been fenced and which Iraq maintained, was for making single cell protein, essentially housed facilities for making biological weapons. In 1995, it was discovered that Iraq had imported 40 tonnes of bacterial growth media in the 1980s which could only be for making biological weapons.
According to the US Defence Department, there are large stocks of Anthrax in Syria, Iran, Libya, China, South and North Korea, Taiwan and Israel. Strangely, it excludes itself and the UK where perhaps the stocks are the largest. In 1944, the US provided funds to produce 275,000 botulin bombs and one million anthrax bombs.
In 2003, the US Government gave $1.5 billion as an additional grant to an institution (NIAID at the National Institutes of Health) to work on selected agents of biological warfare: to develop an enzyme to lyse anthrax bacilli; and to further work on a vaccine that seems to have been developed by a NIAID scientist against Ebola (the vaccine was being tried on monkeys in 2003). In the past seven years, the US has spent more than $57 billion to shore up the American Bioterrorism Defences, stockpiling drugs against biological weapons and networking detection systems in more than 10 cities and preparedness at hospitals.
After the last world war, the US gave immunity to Lt-Gen Shiro Ishia who started work on biological warfare in Japan in 1931, for exchange of 8000 pages of Japanese data. The US had in 1950, large stocks of mosquitoes infected with Yellow Fever, Malaria and Dengue; fleas infected with Plague; and ticks infected with Tularemia.
When a few years ago there was an epidemic of measles in a part of the US, they wondered it if it was an act of biological warfare. However, with the effective systems they have, they traced it to a Romanian girl who unknowingly brought the infection to the country. Unfortunately, we do not have such a system and thus cannot be sure whether the Surat plague or the various episodes of Chikungunya have not been surreptitious acts of biological warfare.
Experts have identified strains of pests which are not known to occur in India in some imported consignments of food. In fact, India is totally unprepared for a biological weapons attack in spite of it now being clear that such an attack has a far greater possibility of occurring in comparison to a nuclear attack, for biological weapons are poor man’s atom bombs.